Coding

Part:BBa_K3192017

Designed by: Simonne Guenette   Group: iGEM19_Virginia   (2019-10-12)


styC

This part contains the sequence for the styC gene, which codes for Styrene oxide isomerase. This is a catalytic enzyme that metabolizes styrene oxide into phenylacetaldehyde. This gene originated from Pseudomonas putida S12, an organism found to be capable of metabolizing styrene. This is done through the opening of the epoxide and subsequent oxidation to yield an aldehyde group. This is the second step to the metabolism of styrene to phenylacetic acid in the styABCDE cluster.1

Pathway of styrene degradation into phenylacetic acid. Obtained from Substrate specificity and reaction mechanism of recombinant styrene oxide isomerase from Pseudomonas putida S121











References:

1) Sazinsky, M., Morrison, E., Kantz, A. & Gassner, G. Structure of the Styrene Monooxygenase Flavin Reductase (SMOB) from Pseudomonas putida S12. (2013). doi:10.2210/pdb4f07/pdb


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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